RealHelix™ Premier qPCR Kit [Green, ROX]은 target DNA에 대한 높은 sensitivity와 specificity로 빠르게 실시간 정량(real-time quantification)할 수 있도록 디자인되었습니다. 특별하게 제조된 buffer 및 antibody-mediated hot-start 중합효소는 primer dimer 형성을 줄여주고, 신뢰도 높은 결과를 얻을 수 있도록 해줍니다. 또한, 편리한 2배 농도의 premix에는 Ab+Taq 중합효소, dNTP, buffer, Mg²⁺, green fluorescent dye, ROX 및 안정제(stabilizer)가 포함되어 있습니다. ROX는 PCR 반응에서 사용되지는 않으며, PCR 외 신호를 normalize하거나 반복 반응에서 baseline을 설정하는데 사용할 수 있습니다.

 

Figure 1. Sensitive detection using RealHelix™ Premier qPCR Kit [Green, Low ROX].

A standard sample of 10-fold serial-diluted genomic DNA was analyzed using the RealHelix™ Premier qPCR Kit for the human glucose-6-phosphate dehydrogenase gene-specific primer set. The accuracy of real-time PCR using the RealHelix™ Premier qPCR Kit is demonstrated by R² value=0.99887. NTC: Negative control.

 

 

 

 

Figure 2. Amplification of target DNA with various G+C contents using RealHelix™ Premier qPCR Kit [Green, Low ROX].

It was evaluated by real-time PCR using 10-fold serial diluted human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, and blue: 0.5 ng/rxn) with each instrument. NTC: Negative control.

 

 

 

 

Figure 3. Comparison of real-time PCR using RealHelix™ Premier qPCR Kit [Green, Low ROX] with other company's related products.

10-fold serial dilutions of the human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, and blue: 0.5 ng/rxn) from whole blood were analyzed using the human HPRT-1 gene-specific primer set. Reactions were run on a real-time PCR instrument, CFX-96 (Bio-Rad, USA). NTC: Negative control.

Storage

• Store below -20 ℃
• Should be stored in a dark place.

 

Shelf life

• 12 months